Identification of viral and atypical bacterial pathogens in children hospitalized with acute respiratory infections in Hong Kong by multiplex PCR assays
Identifieur interne : 002D83 ( Main/Exploration ); précédent : 002D82; suivant : 002D84Identification of viral and atypical bacterial pathogens in children hospitalized with acute respiratory infections in Hong Kong by multiplex PCR assays
Auteurs : R. Y. T. Sung [République populaire de Chine] ; Paul K. S. Chan [République populaire de Chine, Hong Kong] ; Tracy Tsen [République populaire de Chine] ; A. M. Li [République populaire de Chine] ; W. Y. Lam [République populaire de Chine] ; Apple C. M. Yeung [République populaire de Chine] ; E. A. S. Nelson [République populaire de Chine]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2009-01.
Descripteurs français
- KwdFr :
- Bactéries (génétique), Bactéries (isolement et purification), Enfant d'âge préscolaire, Exsudats et transsudats (microbiologie), Exsudats et transsudats (virologie), Femelle, Hong Kong (épidémiologie), Humains, Infections bactériennes (diagnostic), Infections bactériennes (microbiologie), Infections bactériennes (épidémiologie), Infections de l'appareil respiratoire (épidémiologie), Infections de l'appareil respiratoire (étiologie), Maladies virales (diagnostic), Maladies virales (virologie), Maladies virales (épidémiologie), Mâle, Nourrisson, Nouveau-né, Réaction de polymérisation en chaîne (), Saisons, Virus (génétique), Virus (isolement et purification).
- MESH :
- diagnostic : Infections bactériennes, Maladies virales.
- génétique : Bactéries, Virus.
- isolement et purification : Bactéries, Virus.
- microbiologie : Exsudats et transsudats, Infections bactériennes.
- virologie : Exsudats et transsudats, Maladies virales.
- épidémiologie : Hong Kong, Infections bactériennes, Infections de l'appareil respiratoire, Maladies virales.
- étiologie : Infections de l'appareil respiratoire.
- Pascal (Inist)
- MESH :
- Wicri :
- geographic : Hong Kong.
- topic : Enfant, Hospitalisation.
English descriptors
- KwdEn :
- Acute, Bacteria, Bacteria (genetics), Bacteria (isolation & purification), Bacterial Infections (diagnosis), Bacterial Infections (epidemiology), Bacterial Infections (microbiology), Child, Child, Preschool, Exudates and Transudates (microbiology), Exudates and Transudates (virology), Female, Hong Kong, Hong Kong (epidemiology), Hospitalization, Humans, Identification, Infant, Infant, Newborn, Male, Multiplex polymerase chain reaction, Nasopharynx, Polymerase Chain Reaction (methods), Respiratory Tract Infections (epidemiology), Respiratory Tract Infections (etiology), Respiratory disease, Respiratory tract, Samplings, Seasons, Virus Diseases (diagnosis), Virus Diseases (epidemiology), Virus Diseases (virology), Viruses (genetics), Viruses (isolation & purification).
- MESH :
- geographic , epidemiology : Hong Kong.
- diagnosis : Bacterial Infections, Virus Diseases.
- epidemiology : Bacterial Infections, Respiratory Tract Infections, Virus Diseases.
- etiology : Respiratory Tract Infections.
- genetics : Bacteria, Viruses.
- isolation & purification : Bacteria, Viruses.
- methods : Polymerase Chain Reaction.
- microbiology : Bacterial Infections, Exudates and Transudates.
- virology : Exudates and Transudates, Virus Diseases.
- Teeft :
- Adenovirus, Administrative region, Aspirate, Assay, Atypical, Atypical bacteria, Chan, Child, Preschool, Chinese university, Chlamydophila pneumoniae, Clin, Clin microbiol, Clinical manifestations, Coronavirus, Current study, Disease burden, Febrile convulsions, Female, Hong kong, Human coronaviruses, Humans, Infant, Infant, Newborn, Infection, Legionella pneumophila, Male, Metapneumovirus, Microbiol, Multiplex, Mycoplasma, Mycoplasma pneumoniae, Nasal, Nasopharyngeal, Nasopharyngeal aspirate, Pathogen, Pneumoniae, Positive cases, Positive rate, Previous reports, Respiratory pathogens, Respiratory syncytial virus, Respiratory viruses, Rhinovirus, Seasons, Syncytial, Tract infection, Tract infections, Viral, Viral infections, Virol, Virus, Wales hospital.
Abstract
Acute respiratory tract infection is a leading cause of hospital admission of children. This study used a broad capture, rapid and sensitive method (multiplex PCR assay) to detect 20 different respiratory pathogens including influenza A subtypes H1, H3, and H5; influenza B; parainfluenza types 1, 2, 3, and 4; respiratory syncytial virus (RSV) groups A and B; adenoviruses; human rhinoviruses; enteroviruses; human metapneumoviruses; human coronaviruses OC43, 229E, and SARS‐CoV; Chlamydophila pneumoniae; Legionella pneumophila; and Mycoplasma pneumoniae; from respiratory specimens of 475 children hospitalized over a 12‐month period for acute respiratory tract infections. The overall positive rate (47%) was about twice higher than previous reports based on conventional methods. Influenza A, parainfluenza and RSV accounted for 51%, and non‐cultivable viruses accounted for 30% of positive cases. Influenza A peaked at March and June. Influenza B was detected in January, February, and April. Parainfluenza was prevalent throughout the year except from April to June. Most RSV infections were found between February and September. Adenovirus had multiple peaks, whereas rhinovirus and coronavirus OC43 were detected mainly in winter and early spring. RSV infection was associated with bronchiolitis, and parainfluenza was associated with croup; otherwise the clinical manifestations were largely nonspecific. In general, children infected with influenza A, adenovirus and mixed viruses had higher temperatures. In view of the increasing concern about unexpected outbreaks of severe viral infections, a rapid multiplex PCR assay is a valuable tool to enhance the management of hospitalized patients, and for the surveillance for viral infections circulating in the community. J. Med. Virol. 81:153–159, 2009. © 2008 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.21364
Affiliations:
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Y. T." last="Sung">R. Y. T. Sung</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Paediatrics, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Stanley Ho Centre for Emerging Infectious Diseases, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Hong Kong</country></affiliation><affiliation wicri:level="4"><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Correspondence address: Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Tsen, Tracy" sort="Tsen, Tracy" uniqKey="Tsen T" first="Tracy" last="Tsen">Tracy Tsen</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Paediatrics, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Li, A M" sort="Li, A M" uniqKey="Li A" first="A. 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Nelson</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Paediatrics, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">81</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="153">153</biblScope><biblScope unit="page" to="159">159</biblScope><biblScope unit="page-count">7</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2009-01">2009-01</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute</term><term>Bacteria</term><term>Bacteria (genetics)</term><term>Bacteria (isolation & purification)</term><term>Bacterial Infections (diagnosis)</term><term>Bacterial Infections (epidemiology)</term><term>Bacterial Infections (microbiology)</term><term>Child</term><term>Child, Preschool</term><term>Exudates and Transudates (microbiology)</term><term>Exudates and Transudates (virology)</term><term>Female</term><term>Hong Kong</term><term>Hong Kong (epidemiology)</term><term>Hospitalization</term><term>Humans</term><term>Identification</term><term>Infant</term><term>Infant, Newborn</term><term>Male</term><term>Multiplex polymerase chain reaction</term><term>Nasopharynx</term><term>Polymerase Chain Reaction (methods)</term><term>Respiratory Tract Infections (epidemiology)</term><term>Respiratory Tract Infections (etiology)</term><term>Respiratory disease</term><term>Respiratory tract</term><term>Samplings</term><term>Seasons</term><term>Virus Diseases (diagnosis)</term><term>Virus Diseases (epidemiology)</term><term>Virus Diseases (virology)</term><term>Viruses (genetics)</term><term>Viruses (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bactéries (génétique)</term><term>Bactéries (isolement et purification)</term><term>Enfant d'âge préscolaire</term><term>Exsudats et transsudats (microbiologie)</term><term>Exsudats et transsudats (virologie)</term><term>Femelle</term><term>Hong Kong (épidémiologie)</term><term>Humains</term><term>Infections bactériennes (diagnostic)</term><term>Infections bactériennes (microbiologie)</term><term>Infections bactériennes (épidémiologie)</term><term>Infections de l'appareil respiratoire (épidémiologie)</term><term>Infections de l'appareil respiratoire (étiologie)</term><term>Maladies virales (diagnostic)</term><term>Maladies virales (virologie)</term><term>Maladies virales (épidémiologie)</term><term>Mâle</term><term>Nourrisson</term><term>Nouveau-né</term><term>Réaction de polymérisation en chaîne ()</term><term>Saisons</term><term>Virus (génétique)</term><term>Virus (isolement et purification)</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Hong Kong</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Bacterial Infections</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections bactériennes</term><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Bacterial Infections</term><term>Respiratory Tract Infections</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteria</term><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bactéries</term><term>Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Bactéries</term><term>Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Exsudats et transsudats</term><term>Infections bactériennes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Bacterial Infections</term><term>Exudates and Transudates</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Exsudats et transsudats</term><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Exudates and Transudates</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Hong Kong</term><term>Infections bactériennes</term><term>Infections de l'appareil respiratoire</term><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="étiologie" xml:lang="fr"><term>Infections de l'appareil respiratoire</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Aigu</term><term>Bactérie</term><term>Enfant</term><term>Hong Kong</term><term>Hospitalisation</term><term>Identification</term><term>Nasopharynx</term><term>Pathologie de l'appareil respiratoire</term><term>Prélèvement</term><term>Réaction chaîne polymérase multiplex</term><term>Voie respiratoire</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Adenovirus</term><term>Administrative region</term><term>Aspirate</term><term>Assay</term><term>Atypical</term><term>Atypical bacteria</term><term>Chan</term><term>Child, Preschool</term><term>Chinese university</term><term>Chlamydophila pneumoniae</term><term>Clin</term><term>Clin microbiol</term><term>Clinical manifestations</term><term>Coronavirus</term><term>Current study</term><term>Disease burden</term><term>Febrile convulsions</term><term>Female</term><term>Hong kong</term><term>Human coronaviruses</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>Infection</term><term>Legionella pneumophila</term><term>Male</term><term>Metapneumovirus</term><term>Microbiol</term><term>Multiplex</term><term>Mycoplasma</term><term>Mycoplasma pneumoniae</term><term>Nasal</term><term>Nasopharyngeal</term><term>Nasopharyngeal aspirate</term><term>Pathogen</term><term>Pneumoniae</term><term>Positive cases</term><term>Positive rate</term><term>Previous reports</term><term>Respiratory pathogens</term><term>Respiratory syncytial virus</term><term>Respiratory viruses</term><term>Rhinovirus</term><term>Seasons</term><term>Syncytial</term><term>Tract infection</term><term>Tract infections</term><term>Viral</term><term>Viral infections</term><term>Virol</term><term>Virus</term><term>Wales hospital</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Nourrisson</term><term>Nouveau-né</term><term>Réaction de polymérisation en chaîne</term><term>Saisons</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Hong Kong</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Enfant</term><term>Hospitalisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Acute respiratory tract infection is a leading cause of hospital admission of children. This study used a broad capture, rapid and sensitive method (multiplex PCR assay) to detect 20 different respiratory pathogens including influenza A subtypes H1, H3, and H5; influenza B; parainfluenza types 1, 2, 3, and 4; respiratory syncytial virus (RSV) groups A and B; adenoviruses; human rhinoviruses; enteroviruses; human metapneumoviruses; human coronaviruses OC43, 229E, and SARS‐CoV; Chlamydophila pneumoniae; Legionella pneumophila; and Mycoplasma pneumoniae; from respiratory specimens of 475 children hospitalized over a 12‐month period for acute respiratory tract infections. The overall positive rate (47%) was about twice higher than previous reports based on conventional methods. Influenza A, parainfluenza and RSV accounted for 51%, and non‐cultivable viruses accounted for 30% of positive cases. Influenza A peaked at March and June. Influenza B was detected in January, February, and April. Parainfluenza was prevalent throughout the year except from April to June. Most RSV infections were found between February and September. Adenovirus had multiple peaks, whereas rhinovirus and coronavirus OC43 were detected mainly in winter and early spring. RSV infection was associated with bronchiolitis, and parainfluenza was associated with croup; otherwise the clinical manifestations were largely nonspecific. In general, children infected with influenza A, adenovirus and mixed viruses had higher temperatures. In view of the increasing concern about unexpected outbreaks of severe viral infections, a rapid multiplex PCR assay is a valuable tool to enhance the management of hospitalized patients, and for the surveillance for viral infections circulating in the community. J. Med. Virol. 81:153–159, 2009. © 2008 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>République populaire de Chine</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Sung, R Y T" sort="Sung, R Y T" uniqKey="Sung R" first="R. Y. T." last="Sung">R. Y. T. Sung</name></noRegion><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name><name sortKey="Lam, W Y" sort="Lam, W Y" uniqKey="Lam W" first="W. Y." last="Lam">W. Y. Lam</name><name sortKey="Li, A M" sort="Li, A M" uniqKey="Li A" first="A. M." last="Li">A. M. Li</name><name sortKey="Nelson, E A S" sort="Nelson, E A S" uniqKey="Nelson E" first="E. A. S." last="Nelson">E. A. S. Nelson</name><name sortKey="Tsen, Tracy" sort="Tsen, Tracy" uniqKey="Tsen T" first="Tracy" last="Tsen">Tracy Tsen</name><name sortKey="Yeung, Apple C M" sort="Yeung, Apple C M" uniqKey="Yeung A" first="Apple C. M." last="Yeung">Apple C. M. Yeung</name></country><country name="Hong Kong"><noRegion><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Identification of viral and atypical bacterial pathogens in children hospitalized with acute respiratory infections in Hong Kong by multiplex PCR assays
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